BRAFm+ Metastatic melanoma
What makes it different, makes it vulnerable

Importance of the pre-analytical phase steps

The pre-analytical steps take place in the institution where the sample is taken and the closest histopathology laboratory, often in the same institution where the sample is acquired. Handling and pre-treatment of the tissue during transport to the pathology laboratory (see Step 2), as well as within the laboratory, are critical stages that can affect the quality of the tissue, proteins and nucleic acids available for further examination and testing.1

In the time between administration of the anaesthetic to the patient and ligation of the vessels during tumour excision, (in addition to sample handling and transport until fixation), there are changes that occur in gene expression, with degradation of biomolecules (autolysis), due to ischemia of the tissue/cells.2

  • Warm ischemia – during the surgical procedure
  • Cold ischemia – during the sample handling and transport

The tissue preservation procedures, including fixation and paraffin embedding have a significant impact on the quality of the tissue, thereby affecting testing, and must be performed based on accepted standards and recommendations.1–4

Pre-analytical phase of the diagnostic procedure