It is important to understand that any diagnostic test is an integrated set of processes. These include many steps encompassing a number of medical disciplines, conducted by different stakeholders. The available testing techniques are discussed in BRAF mutation testing methods, including new technologies that are replacing traditional Sanger sequencing, the importance of detecting not only BRAF V600E mutations, but other mutations at this site, and the utility of second-line testing of negative samples.
To ensure biomarker testing is clinically useful, the sample should be acquired and prepared appropriately.1 Just as important is the choice of analytical testing method (pre-analytical phase), as well as timely testing and accurate result delivery. All these critical steps require expertise, as well as continual monitoring with both internal and external quality control schemes.1
Here you’ll find a step-by-step guide to the BRAF testing procedure, including information on sample acquisition, transport and preparation for testing (pre-analytical phase), target isolation and mutation detection (analytical phase), test reporting procedures, and quality control recommendations.