The different methods for assessing BRAF mutations can be compared based on:
Below are some of the published results of different expert teams, comparing different methods.
Publication |
Methods compared |
Comparison of targeted next generation sequencing (NGS) versus isolated BRAF V600E analysis in patients with metastatic melanoma2 |
Allele-specific PCR, NGS |
Droplet digital PCR for detection of BRAF V600E mutation in formalin-fixed, paraffin-embedded melanoma tissues: a comparison with Cobas® 4800, Sanger sequencing, and allele-specific PCR3 |
Cobas 4800 BRAF Mutation Test; ddPCR, Sanger sequencing, allele-specific PCR |
Rapid BRAF mutation tests in patients with advanced melanoma: comparison of immunohistochemistry, Droplet Digital PCR, and the Idylla Mutation Platform4 |
Idylla BRAF Mutation Test; IHC, droplet digital PCR (ddPCR), HRM/sequencing |
BRAF V600 mutation detection in melanoma: a comparison of two laboratory testing methods5 |
Ventana Optiview 3’3-DAB IHC detection kit, cobas 4800 BRAF V600 Mutation Test; PCR, IHC |
Comparison of five different assays for the detection of BRAF mutations in formalin-fixed paraffin embedded tissues of patients with metastatic melanoma6 |
Cobas 4800 V600 Mutation Test; HRM, multiplexed RT-ASA, IHC, NGS |
Towards personalized medicine in melanoma: implementation of a clinical next-generation sequencing panel7 |
NGS |
Heterogeneity and frequency of BRAF mutations in primary melanoma: comparison between molecular methods and immunohistochemistry8 |
Real-time PCR, IHC, classical capillary sequencing |
A comparison of immunohistochemical and molecular methods used for analyzing the BRAF V600E gene mutation in malignant melanoma in Taiwan9 |
PCR, IHC |
Detection of BRAF mutations using a fully automated platform and comparison with high resolution melting, real-time allele specific amplification, immunohistochemistry and next generation sequencing assays, for patients with metastatic melanoma10 |
IdyllaTM assay; HRM, real-time allele-specific amplification (RT-ASA), NGS, IHC |
Comparison between two widely used laboratory methods in BRAF V600 mutation detection in a large cohort of clinical samples of cutaneous melanoma metastases to the lymph nodes11 |
Cobas 4800 BRAF V600 Mutation Test, direct Sanger sequencing; PCR/Sanger sequencing, real-time PCR |
Performance comparison of three BRAF V600E detection methods in malignant melanoma and colorectal cancer specimens12 |
LightMix® Kit BRAF V600E/K; IHC, Sanger sequencing, single-probe high-resolution melting assay |
Comparison of molecular testing methods for detecting BRAF V600 mutations in melanoma specimens with challenging attributes13 |
Cobas 4800 BRAF V600 Mutation Test, Qiagen BRAF RGQ PCR kit version 2; real-time PCR |
Comparison of Testing Methods for the Detection of BRAF V600E Mutations in Malignant Melanoma: Pre-Approval Validation Study of the Companion Diagnostic Test for Vemurafenib14 |
Cobas BRAF Mutation Test; ABI BRAF test; and bidirectional direct sequencing |
Comparison of Targeted Next-Generation Sequencing (NGS) and Real-Time PCR in the Detection of EGFR, KRAS, and BRAF Mutations on Formalin-Fixed, Paraffin-Embedded Tumor Material of Non-Small Cell Lung Carcinoma—Superiority of NGS15 |
NGS, real-time PCR |
Detection of BRAF V600 mutations in metastatic melanoma: comparison of the Cobas 4800 and Sanger sequencing assays16 |
Cobas BRAF Mutation Test; Sanger sequencing |
Clinical validation of KRAS, BRAF, and EGFR mutation detection using next-generation sequencing17 |
NGS |
Validation of Next Generation Sequencing Technologies in Comparison to Current Diagnostic Gold Standards for BRAF, EGFR and KRAS Mutational Analysis18 |
NGS, Sanger sequencing, real-time PCR |
Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAF mutations19 |
HRM, NGS, pyrosequencing, IHC, Sanger sequencing |
Comparative evaluation of the new FDA approved THxID™-BRAF test with high resolution melting and sanger sequencing20 |
THxID BRAF test; Real-time PCR, HRM, Sanger sequencing |
KRAS and BRAF mutation analysis in routine molecular diagnostics: comparison of three testing methods on formalin-fixed, paraffin-embedded tumor-derived DNA21 |
HRM, real-time PCR |
Evaluation of BRAF Mutation Testing Methodologies in Formalin-Fixed, Paraffin-Embedded Cutaneous Melanomas22 |
Cobas 4800 BRAF V600 Mutation Test; Sanger sequencing, pyrosequencing, real-time PCR |
Detection of BRAF p.V600E Mutations in Melanomas Comparison of Four Methods Argues for Sequential Use of Immunohistochemistry and Pyrosequencing23 |
IHC, Pyrosequencing, Sanger Sequencing, real-time PCR |
Implementation of a companion diagnostic in the clinical laboratory: The BRAF example in melanoma24 |
Cobas 4800 BRAF V600 Mutation Test, ThxID BRAF test; HRM, real-time PCR, sequencing, NGS |